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The effect of the proteolytic enzyme on the treatment of wool by cutting grinder

Shanghai Tendrew/2017-06-08/Views: 1,667 Views

The study involved wool processing to two stages of keratin hydrolyzate (alkaline pretreatment and enzymatic hydrolysis). The proteolytic enzymes used were Esperase 6.0T and Everlase 6.0T. (OH) 2 (3-9%, w / w), 2, the temperature of the first processing stage (40-80 ℃), the temperature of the first processing stage (40-80 ℃), the temperature of the first processing stage ) 3, the second processing stage temperature (40-60 ℃), were calibrated three influencing factors, divided into 2 ³ models were experiment (respectively, set the minimum and maximum), by breaking the amount of wool for the measurement of the amount of analysis (Determined by weight analysis).

Enzyme Esperase 6.0T, the amount of decomposition wool is 8.4-59.2%;

Enzyme Everlase 6.0 T, decomposed wool amount 4.7-39.6% (depending on the hydrolysis conditions).

The results were statistically analyzed and the statistical significance of the factors was analyzed. The results of the wool breakage are shown graphically on the contour map showing the effect of the two most important factors on the third factor of the selected level and show the way to obtain the desired properties of the keratin hydrolyzate to simulate the fracture conditions possibility.

The predicted experimental measures are:

A:
Ca (OH) 2 concentration 3% -9%

B: the first stage of the temperature of 40 ± 0.5 ℃ ~ 80 ± 0.5 ℃

C: the second stage of the temperature of 40 ± 0.5 ℃ ~ 60 ± 0.5 ℃

The amount of wool decomposition is described by the linear equation: y = k + A · b1 + B · b2 + C · b3 + AB · b12 + BC · b23 + AC · b13

The correlation coefficient R² represents the degree of fitting of the linear equation parameters, and R² = 98.07% and R² = 97.23%, respectively, and the correlation of the linear equation is confirmed.

experiment method:

The conditions of two stages (alkaline pretreatment and alkaline hydrolysis) of wool hydrolysis decomposition were studied using 2 3 types of factor tests.
The effects of three factors on the decomposition of wool were studied. The addition of Ca (OH) 2, the temperature of the first processing stage and the temperature of the second processing stage were studied. When using different proteolytic enzymes Esperase 6.0T and Everlase 6.0T, it was confirmed that there were some differences between wool decomposition efficiency.